Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Low-cost media formulation for culture of brain tumor spheroids (neurospheres).

BioTechniques - 1 Aug 2013

Monterey Michael D, Szerlip Nicholas J, Mathupala Saroj P

Abstract excerpt

Recent studies have found that the biological features of primary tumors are faithfully recapitulated when a patient's tumor is processed and then maintained as a 3-D spheroid in specialized cell culture media. However, a major drawback for maintenance and routine passage of primary tumors as spheroids has been the high cost of custom-formulated media compared to regular serum-supplemented media. Here we report...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Brain Neoplasms
Share to Cell Culture Techniques
Share to Culture Media, Serum-Free

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Glioblastoma

Share to Phenotype

Share to Reproducibility of Results

Share to Spheroids, Cellular

Share to Tumor Cells, Cultured

Source

Open in PubMed Open full text

Identifiers

PMID: 23931597
DOI: 10.2144/000114066
PMCID: PMC3889138

Genes

EGF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

Low-cost media formulation for culture of brain tumor spheroids (neurospheres).PMID 23931597Read ↗

Glioblastoma’s Next Top Model: Novel Culture Systems for Brain Cancer Radiotherapy ResearchSemantic proximity Read ↗

Establishment, Maintenance, and In Vitro and In Vivo Applications of Primary Human Glioblastoma Multiforme (GBM) Xenograft Models for Translational Biology Studies and Drug DiscoverySemantic proximity Read ↗

A standardized and reproducible protocol for serum-free monolayer culturing of primary paediatric brain tumours to be utilized for therapeutic assays.Semantic proximity Read ↗

Establishment and Characterization of Primary Glioblastoma Cell Lines from Fresh and Frozen Material: A Detailed ComparisonSemantic proximity Read ↗

Serum‐free media for the production of human mesenchymal stromal cells: a reviewSemantic proximity Read ↗

Primary glioma spheroids maintain tumourogenicity and essential phenotypic traits after cryopreservation.Semantic proximity Read ↗

Alginate Encapsulation as a Novel Strategy for the Cryopreservation of NeurospheresSemantic proximity Read ↗

A molecularly distinct subset of glioblastoma requires serum‐containing media to establish sustainable bona fide glioblastoma stem cell culturesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.