Article
Typing of hepatitis B virus genomes by a simplified polymerase chain reaction.
Journal of medical virology - 1 Jul 1990
Norder H, Hammas B, Magnius L O
Abstract excerpt
The amplification of hepatitis B virus (HBV) DNA sequences in sera for molecular epidemiology of HBV is an important application of the polymerase chain reaction (PCR) with regard to HBV. To simplify the PCR for this purpose, the optimal concentrations of SDS and detergents for carrying out the p...
Topics
- Base Sequence
- DNA, Viral
- DNA-Directed DNA Polymerase
- Detergents
- Endopeptidase K
- Genes, Viral
- Genotype
- Hepatitis B virus
- Humans
- Molecular Sequence Data
- Nucleic Acid Amplification Techniques
- Phenol
- Phenols
- Polymerase Chain Reaction
- Sequence Homology, Nucleic Acid
- Serine Endopeptidases
- Taq Polymerase
