Article
Genotyping by multiplex polymerase chain reaction for detection of endemic hepatitis B virus transmission.
Journal of clinical microbiology - 1 May 1993
Repp R, Rhiel S, Heermann K H, Schaefer S, Keller C, Ndumbe P, Lampert F, Gerlich W H
Abstract excerpt
A nested polymerase chain reaction (PCR) protocol was developed for rapid genotyping of hepatitis B virus (HBV). During the first PCR round, a universal HBV primer pair was used to amplify the entire pre-S region of the HBV genome. Within the pre-S region, many nucleotide exchanges are observed. These are partly correlated to the serological hepatitis B surface antigen subtypes. Five additional subtype-specific...
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