Article
Application of real-time RT-PCR in vector surveillance and assessment of replication kinetics of an emerging novel ECSA genotype of Chikungunya virus in Aedes aegypti.
Journal of virological methods - 1 Nov 2013
Agarwal Ankita, Singh Anil K, Sharma Shashi, Soni Manisha, Thakur Ashish K, Gopalan N, Parida M M, Rao P V L, Dash Paban K
Abstract excerpt
Chikungunya has emerged as one of the most important arboviral infection of global significance. Expansion of Chikungunya virus endemic areas can be ascribed to naive population, increasing vector population and adaptability of virus to new vector. In this study, a SYBR Green I based quantitative RT-PCR assay was developed. The assay was found to be 10-fold more sensitive than conventional RT-PCR and no cross...
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