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Assessment of factors influencing the Agrobacterium-mediated in planta seed transformation of brinjal (Solanum melongena L.).

Applied biochemistry and biotechnology - 1 Sept 2013

Subramanyam Kondeti, Rajesh Manoharan, Jaganath Balusamy, Vasuki Amirthalingam, Theboral Jeevaraj, Elayaraja Dhandapani, Karthik Sivabalan, Manickavasagam Markandan, Ganapathi Andy

Abstract excerpt

An efficient and reproducible in planta transformation method was developed for brinjal using seed as an explant. The brinjal seeds were infected with Agrobacterium tumefaciens EHA 105 harbouring pCAMBIA 1301-bar plasmid, and the transformants were selected against BASTA®. Several parameters influencing the in planta seed transformation such as pre-culture duration, acetosyringone concentration, surfactants,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 23852797
DOI: 10.1007/s12010-013-0359-z

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