Article
Engineering a high-yield glutathione strain of Hansenula polymorpha using ion beam implantation.
Preparative biochemistry & biotechnology - 1 Jan 2013
Qian Weidong, Fu Yunfang, Cai Changlong
Abstract excerpt
To generate an industrial strain of Hansenula polymorpha capable of yielding greater levels of glutathione (GSH), wild strain H. polymorpha DL-1 cells were mutated using a nitrogen ion beam, a novel mutagen. At an energy level of 20 keV and dose of 2.13 × 10(16) ions/cm(2), H. polymorpha strain 28 (HP28) with a high-yield of GSH was screened. HP28 intracellular GSH levels reached 337.16 mg/L by ion beam...
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