Article
Resonance Raman studies of iron spin and axial coordination in distal pocket mutants of ferric myoglobin.
The Journal of biological chemistry - 25 Jul 1990
Morikis D, Champion P M, Springer B A, Egebey K D, Sligar S G
Abstract excerpt
We have used resonance Raman spectroscopy to study 11 distal pocket mutants and the "wild type" and native ferric sperm whale myoglobin. The characteristic Raman core-size markers v4, v3, v2, and v10 are utilized to assign the spin and coordination state of each sample. It is demonstrated that replacements of the distal and proximal histidines can discriminate against H2O as a sixth ligand and favor a...
Topics
- Animals
- Ferric Compounds
- Mutation
- Myoglobin
- Spectrum Analysis, Raman
- Structure-Activity Relationship
- Whales