Article
Controlling ligand binding in myoglobin by mutagenesis.
The Journal of biological chemistry - 1 Mar 2002
Draghi Federica, Miele Adriana Erica, Travaglini-Allocatelli Carlo, Vallone Beatrice, Brunori Maurizio, Gibson Quentin H, Olson John S
Abstract excerpt
A quadruple mutant of sperm whale myoglobin was constructed to mimic the structure found in Ascaris suum hemoglobin. The replacements include His(E7)-->Gln, Leu(B10)-->Tyr, Thr(E10)--> Arg, and Ile(G8)-->Phe. Single, double, and triple mutants were characterized to dissect out the effects of the individual substitutions. The crystal structures of the deoxy and oxy forms of the quadruple mutant were determined and...
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