Article
A rapid ribosome profiling method elucidates chloroplast ribosome behavior in vivo.
The Plant cell - 1 Jun 2013
Zoschke Reimo, Watkins Kenneth P, Barkan Alice
Abstract excerpt
The profiling of ribosome footprints by deep sequencing has revolutionized the analysis of translation by mapping ribosomes with high resolution on a genome-wide scale. We present a variation on this approach that offers a rapid and cost-effective alternative for the genome-wide profiling of chloroplast ribosomes. Ribosome footprints from leaf tissue are hybridized to oligonucleotide tiling microarrays of the...
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