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Article

Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering

3 Jun 2013

Abstract excerpt

In this study, a simple three-dimensional (3D) suspension culture method for the expansion and cardiac differentiation of human induced pluripotent stem cells (hiPSCs) is reported. The culture methods were easily adapted from two-dimensional (2D) to 3D culture without any additional manipulations. When hiPSCs were directly applied to 3D culture from 2D in a single-cell suspension, only a few aggregated cells were...

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PMID: 23728860
DOI: 10.1002/term.1761

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