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Article

Production of androgenetic diploid loach by cold-shock of eggs fertilized with diploid sperm.

Theriogenology - 15 Jul 2013

Hou Jilun, Fujimoto Takafumi, Yamaha Etsuro, Arai Katsutoshi

Abstract excerpt

Diploid androgenotes were produced without egg irradiation in the loach, Misgurnus anguillicaudatus. Eggs of wild-type diploid females were fertilized with diploid sperm of a neo-tetraploid male and then cold-shock treated at 3 °C (range, ±0.5 °C) for 30 minutes just after fertilization to eliminate the female nucleus. After hatching, ploidy status of the hatched larvae was analyzed by flow cytometry, which...

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Identifiers

PMID: 23602217
DOI: 10.1016/j.theriogenology.2013.03.014

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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