Article
Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish.
BMC biotechnology - 29 Nov 2011
Morishima Kagayaki, Fujimoto Takafumi, Sato Mami, Kawae Ayako, Zhao Yan, Yamaha Etsuro, Arai Katsutoshi
Abstract excerpt
BACKGROUND: Androgenesis (all-male inheritance) is generally induced by means of irradiating the eggs to inactivate the maternal genome, followed by fertilization with normal sperm. In fish, the conventional technique for induced androgenesis has been applied for rapid fixation to traits, recover...
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