Article
A new method for stranded whole transcriptome RNA-seq.
Methods (San Diego, Calif.) - 15 Sept 2013
Miller David F B, Yan Pearlly S, Buechlein Aaron, Rodriguez Benjamin A, Yilmaz Ayse S, Goel Shokhi, Lin Hai, Collins-Burow Bridgette, Rhodes Lyndsay V, Braun Chris, Pradeep Sunila, Rupaimoole Rajesha, Dalkilic Mehmet, Sood Anil K, Burow Matthew E, Tang Haixu, Huang Tim H, Liu Yunlong, Rusch Douglas B, Nephew Kenneth P
Abstract excerpt
This report describes an improved protocol to generate stranded, barcoded RNA-seq libraries to capture the whole transcriptome. By optimizing the use of duplex specific nuclease (DSN) to remove ribosomal RNA reads from stranded barcoded libraries, we demonstrate improved efficiency of multiplexed next generation sequencing (NGS). This approach detects expression profiles of all RNA types, including miRNA...
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