Article
Efficacy of quantitative RT-PCR for detection of the nucleoprotein gene from different porcine rubulavirus strains.
Archives of virology - 1 Sept 2013
Rivera-Benitez José Francisco, García-Contreras Adelfa del Carmen, Reyes-Leyva Julio, Hernández Jesús, Sánchez-Betancourt José Iván, Ramírez-Mendoza Humberto
Abstract excerpt
Blue-eye disease is an emergent viral swine infection caused by porcine rubulavirus (PoRV). We have developed a qRT-PCR method to detect and quantify expression of the nucleoprotein gene for different PoRV strains. The limit of detection for this assay was 10(2) copies of synthetic RNA. Viral RNA from PoRV was detectable at a TCID50 of 0.01. Significant differences were observed between viral RNA quantification...
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