Article
The genetic risk in mice from radiation: an estimate of the mutation induction rate per genome.
Radiation research - 1 Mar 2013
Asakawa Jun-ichi, Kodaira Mieko, Cullings Harry M, Katayama Hiroaki, Nakamura Nori
Abstract excerpt
Restriction Landmark Genome Scanning (RLGS) is a method that uses end-labeled (32)P NotI sites that are mostly associated with coding genes to visualizes thousands of DNA fragments as spots in two-dimensional autoradiograms. This approach allows direct detection of autosomal deletions as spots with half normal intensity. The method was applied to mouse offspring derived from spermatogonia exposed to 4 Gy of X...
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