Article
Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks.
Kidney international - 1 Jun 2013
Boerries Melanie, Grahammer Florian, Eiselein Sven, Buck Moritz, Meyer Charlotte, Goedel Markus, Bechtel Wibke, Zschiedrich Stefan, Pfeifer Dietmar, Laloë Denis, Arrondel Christelle, Gonçalves Sara, Krüger Marcus, Harvey Scott J, Busch Hauke, Dengjel Joern, Huber Tobias B
Abstract excerpt
A thorough characterization of the transcriptome and proteome of endogenous podocytes has been hampered by low cell yields during isolation. Here we describe a double fluorescent reporter mouse model combined with an optimized bead perfusion protocol and efficient single cell dissociation to yield more than 500,000 podocytes per mouse allowing for global, unbiased downstream applications. Combining mRNA and miRNA...
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