Article
Specific detection and quantification of virulent/avirulent Phytophthora infestans isolates using a real-time PCR assay that targets polymorphisms of the Avr3a gene.
Letters in applied microbiology - 1 May 2013
Clément J A J, Baldwin T K, Magalon H, Glais I, Gracianne C, Andrivon D, Jacquot E
Abstract excerpt
Molecular tools that allow intraspecific quantification and discrimination of pathogen isolates are useful to assess fitness of competitors during mixed infections. However, methods that were developed for quantifying Phytophthora infestans are only specific at the species level. Here, we reported a TaqMan-based real-time PCR assay allowing, according to the specificity of the used probes, an accurate...
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