Article
An improved high yield method to obtain microsatellite genotypes from red deer antlers up to 200 years old.
Molecular ecology resources - 1 May 2013
Hoffmann G S, Griebeler E M
Abstract excerpt
Analysis of DNA from older samples, such as museum specimen, is a promising approach to studying genetics of populations and ecological processes across several generations. Here, we present a method for extracting high quality nuclear DNA for microsatellite analysis from antlers of red deer (Cervus elaphus). The genotyping of individuals was based on nine microsatellite loci. Because the amount of DNA found in...
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