Article
A PCR-based assay for discriminating Cervus and Rangifer (Cervidae) antlers with mitochondrial DNA polymorphisms.
Journal of animal science - 1 Jul 2012
Kim Young Hwa, Kim Eung Soo, Ko Byong Seob, Oh Seung-Eun, Ryuk Jin-Ah, Chae Seong Wook, Lee Hye Won, Choi Go Ya, Seo Doo Won, Lee Mi Young
Abstract excerpt
This study describes a method for discriminating Rangifer antlers from true Cervus antlers using agarose gel electrophoresis, capillary electrophoresis, quantitative real-time PCR, and allelic discrimination. Specific primers labeled with fluorescent tags were designed to amplify fragments from the mitochondrial D-loop genes for various Cervus subspecies and Rangifer tarandus differentially. A 466-bp fragment...
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