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Diagnosis of Brugian Filariasis by Loop-Mediated Isothermal Amplification

13 Dec 2012

Abstract excerpt

In this study we developed and evaluated a Brugia Hha I repeat loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Brugia genomic DNA. Amplification was detected using turbidity or fluorescence as readouts. Reactions generated a turbidity threshold value or a clear visual positive within 30 minutes using purified genomic DNA equivalent to one microfilaria. Similar results were obtained...

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PMID: 23272258
DOI: 10.1371/journal.pntd.0001948

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