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Rapid Detection and Identification of <i>Brugia malayi</i> , <i>B. pahangi</i> , and <i>Dirofilaria immitis</i> by High-Resolution Melting Assay

30 Nov 2012

Abstract excerpt

Human lymphatic filariasis is caused by filarial worms such as Brugia malayi for which the major reservoir is domestic cats. However, domestic cats or dogs also carry nonhuman filaria such as Brugia pahangi and Dirofilaria immitis. We have developed a single-tube, real-time PCR with a high-resolution melting (HRM) analysis assay for detection and identification of B. malayi, B. pahangi, and D. immitis in blood...

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PMID: 23199268
DOI: 10.1089/vbz.2012.0971

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