Article
Analysis of human cytomegalovirus secondary envelopment by advanced electron microscopy.
Cellular microbiology - 1 Feb 2013
Schauflinger Martin, Villinger Clarissa, Mertens Thomas, Walther Paul, von Einem Jens
Abstract excerpt
Electron microscopy (EM) allows visualization of viruses in fixed cells with high resolution. High-pressure freezing for sample fixation in combination with freeze substitution and embedding in resin improves significantly the preservation of cellular structures and specifically of membranes. This advancement allows better visualization of human cytomegalovirus (HCMV) morphogenesis occurring at membranes. To...
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