Article
Detection of heterogeneity of Borrelia burgdorferi in ixodes ticks by culture-dependent and culture-independent methods.
Journal of clinical microbiology - 1 Feb 2013
Xu Guang, Wesker Jaime, White Caitlin, Campbell Jason, Reich Nicholas G, Rich Stephen M
Abstract excerpt
Abilities to detect heterogeneity of ospC genotypes of the Lyme disease spirochete Borrelia burgdorferi in the tick vector by in vitro culture (IVC) and direct PCR (dPCR) were compared. IVC failed to detect one-third of the ospC genotypes detected by dPCR. Among IVC results, common ospC genotypes were overrepresented while occurrence of rare genotypes was underestimated.
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