Article
Fungal palaeodiversity revealed using high-throughput metabarcoding of ancient DNA from arctic permafrost.
Environmental microbiology - 1 Apr 2013
Bellemain Eva, Davey Marie L, Kauserud Håvard, Epp Laura S, Boessenkool Sanne, Coissac Eric, Geml Jozsef, Edwards Mary, Willerslev Eske, Gussarova Galina, Taberlet Pierre, Haile James, Brochmann Christian
Abstract excerpt
The taxonomic and ecological diversity of ancient fungal communities was assessed by combining next generation sequencing and metabarcoding of DNA preserved in permafrost. Twenty-six sediment samples dated 16 000-32 000 radiocarbon years old from two localities in Siberia were analysed for fungal ITS. We detected 75 fungal OTUs from 21 orders representing three phyla, although rarefaction analyses suggested that...
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