Article
An ENU mutagenesis screen in zebrafish for visual system mutants identifies a novel splice-acceptor site mutation in patched2 that results in Colobomas.
Investigative ophthalmology & visual science - 13 Dec 2012
Lee Jiwoon, Cox Ben D, Daly Christina M S, Lee Chanjae, Nuckels Richard J, Tittle Rachel K, Uribe Rosa A, Gross Jeffrey M
Abstract excerpt
PURPOSE: To identify recessive mutations affecting development and/or maintenance of the zebrafish visual system. METHODS: A three-generation ENU (N-Nitroso-N-ethylurea)-based forward genetic screen was performed. F3 embryos were screened visually from 1 to 5 days postfertilization (dpf) for ocular abnormalities, and 5 dpf embryos were fixed and processed for cryosectioning, after which eye sections were screened...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.