Polymerase chain reaction based assay to detect allelic loss in human DNA: loss of beta-interferon gene in chronic myelogenous leukemia.

doi:10.1093/nar/18.4.993

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Article

Polymerase chain reaction based assay to detect allelic loss in human DNA: loss of beta-interferon gene in chronic myelogenous leukemia.

Nucleic acids research - 25 Feb 1990

Neubauer A, Neubauer B, Liu E

Abstract excerpt

We have developed a polymerase chain reaction (PCR) based technique to detect allelic loss. In this differential PCR a target gene and a reference gene are coamplified in the same reaction vessel. The ratio of the intensity of the two resultant bands is an indication of relative gene dosage. This procedure is sensitive in that gene copy ratios of 2:1 and 3:2 (reference: target gene) can readily be detected. Using...

Topics

Alleles
Base Sequence
Cell Line
Chromosome Deletion
DNA
DNA, Neoplasm
Electrophoresis, Agar Gel
Female
Humans
Interferon Type I
Leukemia, Myelogenous, Chronic, BCR-ABL Positive

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Nucleic Acid Amplification Techniques

Oligonucleotide Probes

Placenta

Polymerase Chain Reaction

Pregnancy

Source

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Identifiers

PMID: 2315049
DOI: 10.1093/nar/18.4.993
PMCID: PMC330355

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2315049-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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