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FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

1 Jan 2012

Abstract excerpt

BACKGROUND: Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques. METHODS: We analyzed 2,085 18S rRNA gene sequences from the SILVA database for assay design. We generated and quantified plasmid standards using a qPCR-based approach. We...

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PMID: 23136846
DOI: 10.1186/1471-2180-12-255

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