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The molecular revolution in fungal diagnostics: bridging gaps across clinical, agricultural, and environmental mycology

3 Dec 2025

Abstract excerpt

Fungi play essential roles in human health, agriculture, and ecosystems, yet their diversity has long been underestimated due to reliance on morphology and culture. Molecular innovations-DNA barcoding, multilocus and whole-genome sequencing, NGS, and rapid nucleic acid diagnostics (qPCR, LAMP, CRISPR/Cas)-have revolutionized fungal taxonomy and detection. The ITS region now serves as a universal barcode, often...

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PMID: 42305150
DOI: 10.1080/21501203.2025.2569931

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