Analytical thresholds and sensitivity: establishing RFU thresholds for forensic DNA analysis.

doi:10.1111/1556-4029.12008

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Article

Analytical thresholds and sensitivity: establishing RFU thresholds for forensic DNA analysis.

Journal of forensic sciences - 1 Jan 2013

Bregu Joli, Conklin Danielle, Coronado Elisse, Terrill Margaret, Cotton Robin W, Grgicak Catherine M

Abstract excerpt

Determining appropriate analytical thresholds (ATs) for forensic DNA analysis is critical to maximize allele detection. In this study, six methods to determine ATs for forensic DNA purposes were examined and compared. Four of the methods rely on analysis of the baseline noise of a number of negatives, while two utilize the relationship between relative fluorescence unit signal and DNA input in the polymerase...

Topics

Alleles
DNA
DNA Fingerprinting
Electrophoresis, Capillary
Humans
Models, Genetic
Polymerase Chain Reaction
Signal-To-Noise Ratio
Spectrophotometry, Ultraviolet

Source

Open in PubMed

Identifiers

PMID: 23130820
DOI: 10.1111/1556-4029.12008

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