Article
Differentiating microbial forensic qPCR target and control products by electrospray ionization mass spectrometry.
Biosecurity and bioterrorism : biodefense strategy, practice, and science - 1 Jun 2013
Motley S Timothy, Redden Cassie L, Sannes-Lowery Kristin A, Eshoo Mark W, Hofstadler Steven A, Burans James P, Rosovitz M J
Abstract excerpt
Molecular bioforensic research is dependent on rapid and sensitive methods such as real-time PCR (qPCR) for the identification of microorganisms. The use of synthetic positive control templates containing small modifications outside the primer and probe regions is essential to ensure all aspects of the assay are functioning properly, including the primers and probes. However, a typical qPCR or reverse...
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