Article
Whole genome sequencing of phage resistant Bacillus anthracis mutants reveals an essential role for cell surface anchoring protein CsaB in phage AP50c adsorption.
Virology journal - 26 Oct 2012
Bishop-Lilly Kimberly A, Plaut Roger D, Chen Peter E, Akmal Arya, Willner Kristin M, Butani Amy, Dorsey Shakia, Mokashi Vishwesh, Mateczun Alfred J, Chapman Carol, George Matroner, Luu Truong, Read Timothy D, Calendar Richard, Stibitz Scott, Sozhamannan Shanmuga
Abstract excerpt
BACKGROUND: Spontaneous Bacillus anthracis mutants resistant to infection by phage AP50c (AP50R) exhibit a mucoid colony phenotype and secrete an extracellular matrix. METHODS: Here we utilized a Roche/454-based whole genome sequencing approach to identify mutations that are candidates for conferring AP50c phage resistance, followed by genetic deletion and complementation studies to validate the whole genome...
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