Article
Individual identification of racehorses from urine samples using a 26-plex single-nucleotide polymorphism assay.
Journal of forensic sciences - 1 Jan 2013
Kakoi Hironaga, Kijima-Suda Isao, Gawahara Hitoshi, Kinoshita Kenji, Tozaki Teruaki, Hirota Kei-ichi, Yoshizawa Midori
Abstract excerpt
To construct a system for identifying individual horses from urine samples that are submitted for postracing doping tests, we developed a genotyping assay based on 26-plex single-nucleotide polymorphisms (SNPs). DNA was isolated from urine using a commercially available DNA/RNA extraction kit, and SNP genotyping was achieved with a SNaPshot(™) technique. DNA profiles including 26 SNPs were acquired from urine...
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