Article
Development of a 25-plex SNP assay for traceability in cattle.
Animal genetics - 1 Jun 2009
Karniol B, Shirak A, Baruch E, Singrün C, Tal A, Cahana A, Kam M, Skalski Y, Brem G, Weller J I, Ron M, Seroussi E
Abstract excerpt
Single nucleotide polymorphisms (SNPs) are amenable to automation and therefore have become the marker of choice for DNA profiling. SNaPshot, a primer extension-based method, was used to multiplex 25 SNPs that have been previously validated as useful for identity control. Detection of extended products was based on four different fluorochromes and extension primers with oligonucleotide tails of differing lengths,...
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