Article
Development of a multiple bulked segregant analysis (MBSA) method used to locate a new stem rust resistance gene (Sr54) in the winter wheat cultivar Norin 40.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Feb 2013
Ghazvini Habibollah, Hiebert Colin W, Thomas Julian B, Fetch Thomas
Abstract excerpt
An important aspect of studying putative new genes in wheat is determining their position on the wheat genetic map. The primary difficulty in mapping genes is determining which chromosome carries the gene of interest. Several approaches have been developed to address this problem, each with advantages and disadvantages. Here we describe a new approach called multiple bulked segregant analysis (MBSA). A set of 423...
Topics
- Basidiomycota
- Chromosome Mapping
- Chromosome Segregation
- Chromosomes, Plant
- Disease Resistance
- Gene Expression Regulation, Plant
- Genes, Plant
- Genetic Markers
- Immunity, Innate
- Microsatellite Repeats
- Microscopy, Electron, Scanning Transmission
- Phenotype
- Plant Diseases