Article
Real-time PCR genotyping of Neisseria gonorrhoeae isolates using 14 informative single nucleotide polymorphisms on gonococcal housekeeping genes.
The Journal of antimicrobial chemotherapy - 1 Feb 2013
Whiley David M, Goire Namraj, Rahimi Frashta, Lahra Monica M, Limnios Athena E, Nissen Michael D, Sloots Theo P
Abstract excerpt
OBJECTIVES: Neisseria gonorrhoeae multilocus sequence typing (MLST) is a key tool used to investigate the macroepidemiology of gonococci exhibiting antimicrobial resistance (AMR). However, the utility of MLST is undermined by the high workload and cost associated with DNA sequencing of seven housekeeping genes. In this study, we investigated single nucleotide polymorphism (SNP)-based profiling as a means of...
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