Article
Two distinct sites are essential for virulent infection and support of variant satellite RNA replication in spontaneous beet black scorch virus variants.
The Journal of general virology - 1 Dec 2012
Xu Jin, Wang Xianbing, Shi Lindan, Zhou Yuan, Li Dawei, Han Chenggui, Zhang Ziding, Yu Jialin
Abstract excerpt
Spontaneous point mutations of virus genomes are important in RNA virus evolution and often result in modifications of their biological properties. Spontaneous variants of beet black scorch virus (BBSV) and its satellite (sat) RNA were generated from cDNA clones by serial propagation in Chenopodium amaranticolor and Nicotiana benthamiana. Inoculation with recombinant RNAs synthesized in vitro revealed BBSV...
Topics
- 3' Untranslated Regions
- Base Sequence
- Beta vulgaris
- Chenopodium
- Genetic Variation
- Mutagenesis, Site-Directed
- Plant Diseases
- RNA, Satellite
- RNA-Dependent RNA Polymerase
- Nicotiana
- Tobacco necrosis satellite virus
- Tombusviridae
- Virulence