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Article

Isolation of microsatellite markers for Pelophylax nigromaculata and a tentative application in detecting interspecific introgression.

Gene - 15 Oct 2012

Du Jie, Yan Jie, Zhou Kaiya

Abstract excerpt

Twenty-seven microsatellite markers were obtained from the black-spotted frog, Pelophylax nigromaculata,using the FIASCO (Fast Isolation by AFLP of Sequences containing repeats) protocol. Genotyping of 60 individuals showed that the number of alleles ranged from 4 to 25 with an average of 12.78 alleles per locus. The observed and expected heterozygosities ranged from 0.137 to 0.956 and 0.594 to 0.948, with...

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PMID: 22967873
DOI: 10.1016/j.gene.2012.07.046

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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