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Article

Development of microsatellite markers in Pungtungia herzi using next-generation sequencing and cross-species amplification in the genus Pseudopungtungia.

International journal of molecular sciences - 1 Oct 2013

Yun Young-Eun, Yu Jeong-Nam, Kim Sang Ki, Hwang Ui Wook, Kwak Myounghai

Abstract excerpt

Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain reaction amplification. Of these primer sets, 16 new markers (16%) were successfully amplified with unambiguous...

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PMID: 24084733
DOI: 10.3390/ijms141019923
PMCID: PMC3821594

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