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Metagenomics and metatranscriptomics: Windows on CF-associated viral and microbial communities

28 Aug 2012

Abstract excerpt

BACKGROUND: Samples collected from CF patient airways often contain large amounts of host-derived nucleic acids that interfere with recovery and purification of microbial and viral nucleic acids. This study describes metagenomic and metatranscriptomic methods that address these issues. METHODS: Microbial and viral metagenomes, and microbial metatranscriptomes, were successfully prepared from sputum samples from...

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PMID: 22951208
DOI: 10.1016/j.jcf.2012.07.009

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