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Article

Effects of a buried cysteine-to-serine mutation on yeast triosephosphate isomerase structure and stability.

International journal of molecular sciences - 1 Jan 2012

Hernández-Santoyo Alejandra, Domínguez-Ramírez Lenin, Reyes-López César A, González-Mondragón Edith, Hernández-Arana Andrés, Rodríguez-Romero Adela

Abstract excerpt

All the members of the triosephosphate isomerase (TIM) family possess a cystein residue (Cys126) located near the catalytically essential Glu165. The evolutionarily conserved Cys126, however, does not seem to play a significant role in the catalytic activity. On the other hand, substitution of this residue by other amino acid residues destabilizes the dimeric enzyme, especially when Cys is replaced by Ser. In...

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Identifiers

PMID: 22949845
DOI: 10.3390/ijms130810010
PMCID: PMC3431843

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Source receipt: pubmed, retrieved 7 Sept 2026.

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