Glutamate-194 to cysteine mutation inhibits fast light-induced proton release in bacteriorhodopsin.

doi:10.1021/bi970744y

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Article

Glutamate-194 to cysteine mutation inhibits fast light-induced proton release in bacteriorhodopsin.

Biochemistry - 22 Jul 1997

Balashov S P, Imasheva E S, Ebrey T G, Chen N, Menick D R, Crouch R K

Abstract excerpt

Substitution of glutamic acid-194, a residue on the extracellular surface of bacteriorhodopsin, with a cysteine inhibits the fast light-induced proton release that normally is coupled with the deprotonation of the Schiff base during the L to M transition. Proton release in this mutant occurs at t...

Topics

Bacteriorhodopsins
Cysteine
Dark Adaptation
Glutamic Acid
Halobacterium
Hydrogen-Ion Concentration
Kinetics
Light
Mutation
Protons
Spectrophotometry, Atomic

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