Article
Analysis of genetic variability in human respiratory syncytial virus by the RNase A mismatch cleavage method: subtype divergence and heterogeneity.
Virology - 1 Jan 1990
Cristina J, López J A, Albó C, García-Barreno B, García J, Melero J A, Portela A
Abstract excerpt
We have applied the RNase A mismatch cleavage method to the analysis of genetic variability among human Respiratory Syncytial (RS) viruses. Antisense RNA probes of the Long strain were hybridized to total RNA extracted from cells infected with other strains. The RNA:RNA heteroduplexes were digest...
Topics
- Cell Line
- Cloning, Molecular
- DNA, Viral
- Genetic Variation
- Humans
- Nucleic Acid Hybridization
- Plasmids
- RNA Probes
- RNA, Viral
- Respiratory Syncytial Viruses
- Restriction Mapping
- Ribonuclease, Pancreatic
