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Article

Geographical clusters of dengue virus type 2 isolates based on analysis of infected cell RNA by the chemical cleavage at mismatch method.

Journal of virological methods - 1 Nov 1992

Lin B, Cotton R G, Trent D W, Wright P J

Abstract excerpt

Genetic variation in 12 strains of dengue virus type 2, isolated from several epidemic areas in different years, was studied by chemical cleavage at mismatched cytosine in DNA:RNA heteroduplexes. End-labelled cDNA probes derived from the E and NS2A genes of the New Guinea C strain were hybridized to total RNA extracted from cells infected by individual isolates. Following modification of mismatched cytosine by...

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PMID: 1452634
DOI: 10.1016/0166-0934(92)90069-p

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