Article
Nuclear morphometric analysis (NMA): screening of senescence, apoptosis and nuclear irregularities.
PloS one - 1 Jan 2012
Filippi-Chiela Eduardo C, Oliveira Manuel M, Jurkovski Bruno, Callegari-Jacques Sidia Maria, da Silva Vinicius Duval, Lenz Guido
Abstract excerpt
Several cellular mechanisms affect nuclear morphology which can therefore be used to assess certain processes. Here, we present an analytic tool to quantify the number of cells in a population that present characteristics of senescence, apoptosis or nuclear irregularities through nuclear morphometric analysis. The tool presented here is based on nuclear image analysis and evaluation of size and regularity of...
Topics
- Annexins
- Apoptosis
- Caspase Inhibitors
- Caspases
- Cell Line, Tumor
- Cell Nucleus
- Cellular Senescence
- Cisplatin
- Colonic Neoplasms
- Etoposide
- HeLa Cells
- Humans
- Mitosis
- Phenotype
- Principal Component Analysis