Detection of methicillin resistance in staphylococci by using a DNA probe.

doi:10.1128/AAC.34.9.1720

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Article

Detection of methicillin resistance in staphylococci by using a DNA probe.

Antimicrobial agents and chemotherapy - 1 Sept 1990

Archer G L, Pennell E

Abstract excerpt

A DNA probe derived from the PBP 2a gene of the methicillin-resistant Staphylococcus aureus COL was compared with phenotypic microbiologic tests for its ability to identify methicillin-resistant and -susceptible staphylococci. Lysates were applied to nitrocellulose with a dot blot apparatus. Isolates tested were both S. aureus and coagulase-negative staphylococci that had been recovered from a variety of...

Topics

Bacterial Proteins
Carrier Proteins
Coagulase
DNA Probes
Hexosyltransferases
Immunoblotting
Methicillin Resistance
Multienzyme Complexes
Muramoylpentapeptide Carboxypeptidase
Nucleic Acid Hybridization
Penicillin-Binding Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Peptidyl Transferases

Phenotype

Predictive Value of Tests

Reproducibility of Results

Staphylococcus

Staphylococcus aureus

Source

Open in PubMed Open full text

Identifiers

PMID: 2285284
DOI: 10.1128/AAC.34.9.1720
PMCID: PMC171912

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2285284-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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