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Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction

1 Oct 1991

Abstract excerpt

A simple and reliable method using a polymerase chain reaction (PCR) was devised to identify methicillin-resistant staphylococci. By using lysates of the strain to be tested as templates and 22-mer oligonucleotides as primers, a 533-bp region of mecA, the structural gene of a low-affinity penicillin-binding protein (PBP 2'), was amplified by PCR and detected by agarose gel electrophoresis. Results obtained by...

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PMID: 1939577
DOI: 10.1128/jcm.29.10.2240-2244.1991

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