Genotypic identification of methicillin-resistant coagulase-negative staphylococci by polymerase chain reaction.

doi:10.1128/AAC.35.12.2568

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Article

Genotypic identification of methicillin-resistant coagulase-negative staphylococci by polymerase chain reaction.

Antimicrobial agents and chemotherapy - 1 Dec 1991

Predari S C, Ligozzi M, Fontana R

Abstract excerpt

A rapid method for the detection of methicillin resistance in staphylococci was developed. The method was based on the polymerase chain reaction (PCR) and primers that targeted the internal region of the coding frame of the mec gene. The amplification reaction was carried out with crude cell lysa...

Topics

Coagulase
DNA, Bacterial
Genotype
Hybridization, Genetic
Methicillin
Methicillin Resistance
Phenotype
Polymerase Chain Reaction
Staphylococcus aureus

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