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Degradation of chloroaromatics: structure and catalytic activities of wild-type chlorocatechol 2,3-dioxygenases and modified ones.

Environmental microbiology - 1 Jan 2013

Schmidt Eberhard, Mandt Christian, Janssen Dick B, Pieper Dietmar H, Reineke Walter

Abstract excerpt

To improve the efficiency and to investigate the molecular determinants that direct substrate specificity of chlorocatechol 2,3-dioxygenase CbzE(GJ31) , several mutant enzymes were constructed. Loci for substitutions of amino acids were selected by sequence comparisons as well as by homology modelling of known chlorocatechol 2,3-dioxygenases (CbzE(BASF) , CbzE(SK1) and CbzE(16-6A)). Activity measurements with...

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PMID: 22816508
DOI: 10.1111/j.1462-2920.2012.02831.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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