Construction of plasmid-free derivatives of Salmonella typhimurium LT2 using temperature-sensitive mutants of pKZ1 for displacement of the resident plasmid, pSLT.

doi:10.1007/BF00633852

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Article

Construction of plasmid-free derivatives of Salmonella typhimurium LT2 using temperature-sensitive mutants of pKZ1 for displacement of the resident plasmid, pSLT.

Molecular & general genetics : MGG - 1 Jul 1990

Kelln R A, Lintott L G

Abstract excerpt

Replication (or partitioning) temperature-sensitive mutants of pKZ1 were isolated and shown to exhibit incompatibility with the resident plasmid (pSLT) of Salmonella typhimurium LT2. Following displacement of pSLT, the mutant plasmids were effectively eliminated from the cell population by passag...

Topics

Blotting, Southern
Conjugation, Genetic
DNA, Bacterial
Kanamycin Resistance
Mutation
Plasmids
Salmonella typhimurium
Temperature
Transformation, Bacterial

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