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Article

HypF, a carbamoyl phosphate-converting enzyme involved in [NiFe] hydrogenase maturation.

The Journal of biological chemistry - 20 Dec 2002

Paschos Athanasios, Bauer Anette, Zimmermann Anja, Zehelein Eva, Böck August

Abstract excerpt

HypF has been characterized as an auxiliary protein whose function is required for the synthesis of active [NiFe] hydrogenases in Escherichia coli and other bacteria. To approach the functional analysis, in particular the involvement in CO/CN ligand synthesis, HypF was purified from an overproducing strain to apparent homogeneity. The purified protein behaves as a monomer on size exclusion chromatography, and it...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 12377778
DOI: 10.1074/jbc.M204601200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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HypF, a carbamoyl phosphate-converting enzyme involved in [NiFe] hydrogenase maturation.PMID 12377778Read ↗

Maturation of the [NiFe] hydrogenases.Semantic proximity Read ↗

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[FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation.Semantic proximity Read ↗

Interaction between hydrogenase maturation factors HypA and HypB is required for [NiFe]-hydrogenase maturation.Semantic proximity Read ↗

CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase.Semantic proximity Read ↗

Metal binding activity of the Escherichia coli hydrogenase maturation factor HypB.Semantic proximity Read ↗

Duplication of hyp genes involved in maturation of [NiFe] hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

Transcriptomic profiling and its implications for the H2 production of a non-methanogen deficient in the frhAGB-encoding hydrogenase.Semantic proximity Read ↗

Evidence for an oxygen-sensitive iron-sulfur cluster in an immature large subunit species of Escherichia coli [NiFe]-hydrogenase 2.Semantic proximity Read ↗

N-terminal truncations in the FhlA protein result in formate- and MoeA-independent expression of the hyc (formate hydrogenlyase) operon of Escherichia coli.Semantic proximity Read ↗

Involvement of hyp gene products in maturation of the H(2)-sensing [NiFe] hydrogenase of Ralstonia eutropha.Semantic proximity Read ↗

[FeFe] hydrogenases and their evolution: a genomic perspective.Semantic proximity Read ↗

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